CytoSMART Lux2

Cell Culture Video
Monitoring

  • CytoSMART Lux2

CytoSMART Lux2

Life sciences researchers are gathering more and more information on cellular mechanisms and cell and tissue culture techniques. Understanding cell culture behavior and evaluating cellular health requires extensive training and experience. Processes like cell subculturing, growth rates and nutrition depletion require an experts intuition and trial-and-error based optimization. To communicate these findings is not straightforward. Personal teaching is ideal, but in many cases not manageable. As such video monitoring of cell cultures can be highly beneficial to showcase the progression of cell cultures in an intuitive manner.

The CytoSMART Lux2 provides an easy and cost-effective solution for time-lapse imaging. Monitor cell proliferation, motility and morphology for hours up to weeks at a time. The CytoSMART Lux2 is a compact inverted lab microscope for bright-field live cell imaging. It works from inside any standard CO2-incubator and can be used in routine cell culture processes.

Easy to use. Great instrument.

Pooja Sharma Catholic University of America

"I have used this instrument for live cell imaging. The results are reproducible. The instrument is easy to use and you can remotely access your data."

4.7
Application Area: Live cell imaging

Very good instrument for long term imaging.

Neshika Wijewardhane King's College London

"Very easy product to use, basically plug it in and it works. Love that it can be used inside an incubator as I had trouble with long term imaging with on stage incubators for big microscopes. Also, don't need to worry about fluctuations in temperature and oxygen percentages and deal with oxygen tanks. I would like there to be different magnifications and possibly brightfield, but it isn't essential."

5.0
Application Area: Long term live imaging

Great in all senses.

Fidel Ovidio Castro Universidad de Concepción

"Easy to handle, reliable and produces good images. Its a good bargain."

4.3
Application Area: Cell culture

Great to use, makes life easier in cell culture.

Bianca Pachane UFSCar (BR)

"The equipment is very straightforward to use and has a clean layout. So far, we have had no issues regarding its use."

5.0
Application Area: Cell culture visualization and proliferation

Good for time lapses.

Jesús Peña UC Riverside

"I used the CytoSMART Lux2 to observe fungi. The contrast was great, I was able to track hyphae over a couple days before it became a tangled mess. Cloud storage was very handy. I liked the minimalist design, made it very simple to operate. Would use again!"

5.0
Application Area: 4.7

Observe your cells from your PC, tablet and smartphone!

Lorenzo Depau University of Siena

""CytoSMART Lux2 is a truly useful product in the laboratory. It allows you to obtain real-time images of your experiments and can also be used for time-lapse monitoring of cell cultures. It is very easy to use thanks to the integrated tablet and can be considered a low cost solution to start cell migration studies."

4.7
Application Area: Live cell analysis, cell migration

The cells can be easily tracked by this machine.

Shiro Suetsugu NAIST

"We use this device to monitor cell proliferation and cell migration. It gives nice and easy data in the incubator. The cells can be monitored even with a smartphone."

5.0
Application Area: Cell migration and proliferation

Live insight in cell health and growth rates

Automated live cell microscopy with a remote connection

Culturing cells can be a black-box experiment. In between inspection rounds assumptions are made of cell growth rates and cell viability based on the researchers experience. Using the CytoSMART Lux2, these assumptions are redundant as time-lapse movies are automatically generated and can be assessed immediately. Obtain real-time updates of the health of your cells, even during image acquisition.

Online connection to inspect your cell cultures

Remote access helps to decide when it's time to go to the lab

CytoSMART’s compact cell video monitoring system can film living cells without disturbing their growth. The device is accessible from an online environment, thus enabling researchers to look at their cell cultures and assess what the next steps are, without having to step into the laboratory.

Perfect timing of subculturing

Use the CytoSMART Lux2 confluence notification service

Using cell monitoring software, users can set alerts for target confluence levels. You will receive a notification email once your cell culture has reached the set confluence and is ready for subculture or experiments, such as transfection. The notification system uses CytoSMART’s cloud-based image analysis algorithms. Recorded images are analyzed in real-time to determine the confluence level (% occupied area), providing insight in proliferation rates.

Very useful and convenient to check your cells, especially for the weekends.

Alejandro Rodriguez Ruiz | PhD-candidate

Leiden University Medical Center

Very useful and convenient to check your cells, especially for the weekends.

Want to know what is happening to your cells?

Morphology, Migration, Cytotoxicity, Proliferation and more.

This live cell microscope creates images and movies that contain many cellular features, which can be extracted using image analysis tools.


Monitor cellular behavior over time and investigate how cells respond and interact with their environment. See our overview of applications and assays that can be performed using the CytoSMART Lux2.

Monitor cells in their optimized environment

Easily fits in your incubator

Dependent on growth speed and experimental conditions, cell cultures progress over periods of hours, days or even weeks. To maintain cell health, or to perform biological assays, cells should be kept at the required environmental conditions throughout the entire experiment. The CytoSMART Lux2 is compact, its size is comparable to a large culture flask, so it comfortably fits inside any standard CO2- or hypoxia incubator, ensuring your cells will remain at the desired environmental conditions.

Use the appropriate culture vessel for your cells or experiment

Monitor cells inside flasks, microfluidic chips and more

The open design of the CytoSMART Lux2 allows users to image any transparent culture vessel, including culture flasks, well plates, petri dishes and microfluidic chips. It’s rapid recording interval makes the CytoSMART Lux2 extremely suitable for live-cell imaging of cells cultured inside lab-on-a-chip platforms or micro Total Analysis Systems (µTAS). The CytoSMART Lux2 can be used to investigate organ-on-a-chip systems, the effect of fluid flow on cells or single cell migration inside a chip.

Packages

The CytoSMART Lux2 can be configured in multiple packages.

Lux2 Duo Kit package

Perform comparison studies with live cell imaging

The CytoSMART Lux2 Duo Kit is a compact automated system, specifically designed to operate from within CO2- incubators and hypoxia chambers. Two devices operate via a single laptop, saving precious lab space.

CytoSMART Multi Lux package

Run long-term non-invasive, label-free image analysis

Multi Lux is a cost-effective solution for researchers who want to carry out side-by-side comparisons between cell cultures, run long-term experiments, and monitor cells from their home’s comfort.

Technical specifications

Optics Bright-field only with digital phase contrast
Magnification 10x fixed objective
Fluorescence Filters N/A
Camera 5MP CMOS
Image Formats JPG, XLSX and MP4
Image Size 960 x 960 pixels
Field of View 1.84 x 1.84 mm
Culture well-plates, petri dishes, flasks, microfluidic chips and custom culture vessels
Computer Requirements Windows 10
Power Supply AC 100-240V, 2A, 10W, 50/60HZ
Dimensions 13.3 x 9.0 x 10.0 cm (L x W x H)
5.2 x 3.5 x 3.9 in (L x W x H)
Weight 0.5 kg (1.1 lb)
Operating Conditions 0 - 42 °C, 5-95% humidity
Warranty 1 year parts & labor
Research use only. Not intended for diagnostic purposes

Appnotes

Cell Culture Monitoring - Lux2

Examples of the Effects of Environment Control and Confluency on Cell Cultures
Download Appnote

Cell Motility Video Monitoring - Lux2

Follow Tube Formation and Cell Migration using the CytoSMART Lux2
Download Appnote

Frequently Asked Questions

The CytoSMART Lux2 is a compact inverted microscope that is designed to be placed inside an incubator to monitor cell cultures. The device can be used as a notification system to alert users when a cell culture confluence target is reached.

No calibration by the user is needed for the device to operate.

The device can be used with a Windows based tablet or PC. A WiFi or Ethernet internet connection is required to be able to connect to the CytoSMART server for data storage and analysis.

The CytoSMART Lux2 allows monitoring of a wide range of different culture dishes and flasks, such as:


  • ​T-flasks: T-25 up to T-250
  • Single well, multi-well plates (6, 12, 24, 96 well)
  • microfluidic chips
  • Flat tubes
  • Petri dishes
  • Slides

For larger flasks an adapter plate may be required. This plate can be purchased at CytoSMART.

The CytoSMART Lux2 has a magnification equal to a microscope with a 10X and 20X lens.

The CytoSMART Lux2 records images at predefined intervals. At the start of a new project you can specify the interval rate from an image every 5 minutes to every 12 hours

The device is easy to clean using lint-free swipes and EtOH (70%) or IPA. Do not use Acetone to clean the device, also the device cannot be autoclaved.

After sterilizing with EtOH or IPA the device can be used in a clean room.

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