Cancer spheroids and tumoroids are versatile three-dimensional (3D) in vitro models generated from cancer cells and tumor tissues. These 3D cancer models are more representative of in vivo tumor biology and cellular interactions, as compared to traditional 2D cell cultures. For that reason, scientists are using them to evaluate candidate immune cell products and accelerate preclinical drug development for urgently needed cellular immunotherapies. The CytoSMART platforms make it easy to study the formation, growth, and health of cancer spheroids in real time.
With 3D in vitro cancer spheroid assays:
- >> Track spheroid growth and death in real time
- >> Investigate immunotherapy potency and drug response using non-invasive viability and cytotoxicity analysis
- >> Examine solid tumor heterogeneity
Track spheroid growth and death in real time
Deriving physiologically relevant information about tumor spheroid formation, morphology, and health is essential for the development of effective cancer treatments. As spheroids grow under optimal conditions inside your incubator, they can be non-invasively monitored using the CytoSMART Omni and Lux platforms to study spheroid development and assess the potency of therapeutic agents.
Here, two types of cancer cells – GFP-transfected HeLa cells and C6 cells stained with CellTracker Orange – were co-cultured and monitored using the CytoSMART Omni FL for 72 hours. Within the first 24 hours, cancer spheroid formation was observed.
>> Assay your cells in brightfield and fluorescence – From label-free cell monitoring to fluorescence-based assays, add dynamic visual data in any experiment.
>> Maintain the optimal culture environment – The CytoSMART Omni and Lux platforms operate within an incubator.
>> Track every moment – Automatically capture images as your cells grow in their optimal environment.
>> Get started quickly – The CytoSMART live-cell analysis platforms are designed to be simple and flexible. With a host of analysis modules available, it is easy to adapt the systems to meet your needs.