Authors: Mitdank H, Tröger M, Sonntag A, Shirazi NA, Woith E, Fuchs H, Kobelt D, Walther W, Weng A.
European Journal of Pharmaceutical Sciences, Volume 170, 2022
Here, Mitdank and colleagues designed novel suicide nanoplasmid constructs encoding plant-derived ribosome-inactivating proteins with the aim of providing evidence of the suitability of such nanocomplexes for anti-tumor treatment in vivo. The CytoSMART Omni live-cell imaging device was used to measure the cytotoxic effect of suicide nanoplexes on murine neuroblastoma cells Neuro2A and Neuro2A cells that were transfected with the luciferase gene. In addition, the cell lines were also transfected with eGFP-nanoplexes and the fluorescence live-cell imager, CytoSMART Lux3 FL, was used to monitor cell growth and eGFP expression during the 48-hour period. Overall, it was shown that targeted suicide nanoplexes harboring novel nanoplasmids demonstrate anti-tumoral activity in neuroblastoma cells.
