Authors: Manford AG, Mena EL, Shih KY, Gee CL, McMinimy R, Martínez-González B, Sherriff R, Lew B, Zoltek M, Rodríguez-Pérez F, Woldesenbet M, Kurian J, Rape M.
Cell Press Volume 184, Issue 21, 2021
In this study the Exact (Corning Cell Counter) was used to count and analyze the proliferation of HEK293T cells, expressing variants of FEM1B, which is a component of an E3 ubiquitin-protein ligase complex. Overall, this paper highlights the importance of the key reductive stress response involving the E3 ligase CUL2FEM1B that selectively ubiquitylates the mitochondrial gatekeeper FNIP1. In turn, this activates oxidative phosphorylation and thereby allows cells to replenish their ROS and, likely, ATP supplies.
