Authors: Pelgrim CE, Ark I, Leusink-Muis T, Brans MAD, Braber S, Garssen J, Helvoort A, Kraneveld AD, Folkerts G.
Journal of Pharmacological and Toxicological Methods, Volume 114, 2022
The authors of this study describe an optimized intubation-mediated intratracheal administration (IMIT) technique, as well as compare it to oropharyngeal aspiration (OA). The Exact (Corning Cell Counter) was utilized to determine the total bronchoalveolar lavage (BAL) cell counts of mice treated with increasing doses of lipopolysaccharide (LPS) or saline to compare efficacy of IMIT and OA techniques. All in all, it was demonstrated that the optimized IMIT is superior to OA with regards to efficacy, reproducibility and accuracy.
