Author: Rea J, Carissimo A, Trisciuoglio D, Illi B, Picard D, Remke M, Laneve L, Caffarelli E.
Cancers, 2021
The authors of this study combined transcriptomics and revererse genetics approaches to identify the long noncoding RNAs (lncRNAs) that are implicated in Group 3 Medulloblastoma. They used the CytoSMART Exact (formerly known as the Corning Cell Counter) to seed the D283 MED cells in the desired concentration and perform a viability test using tryphan blue. Alltogether, they found three lncRNAs that are directly involved in the phenotypes of Medulloblastoma cancer cell phenotypes.