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Seeing is believing: label-free, time-lapse microscopy for quantifying cell proliferation

Cell proliferation assays are used in a wide variety of contexts. For example, proliferation can be used to gauge the health of new transgenic cell lines or to evaluate the success of tissue regeneration experiments. Quantitative cell proliferation assays are also essential for evaluating the dose-response of new pharmaceutical agents, such as in the context of anti-cancer drug development.

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Optical microscopy vs. digital microscopy: for cell cultures studies

A variation of the optical microscope is the digital microscope. These microscopes use a digital camera instead of eyepieces. Images are seen in real-time on a computer screen. The invention of the USB port in the 1990s has made available a wide variety of USB digital microscopes to suit every need.

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CYTOSMART ACADEMY

Issues when using human umbilical cord mesenchymal stem cells (hUC MSCs) - CytoSMART Academy

There is a number of issues associated with using mesenchymal stem cells derived from the umbilical cord. The isolation and culture of these cells take a long time - from several days up to several weeks. Once enough cells have been isolated, they need to be expanded to achieve the desired cell concentration. Because this process can take up to several weeks, it needs more handling steps (e.g. medium change, cell monitoring), which increases the chances of culture contamination. In addition, the stem cell culture cannot get too confluent or it will lose its potential. The cells will also start to differentiate into (unwanted) cell types or stop proliferating at all. If this happens, all the time and work will have been in vain.

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RESOURCES

Remote monitoring of iPSC-derived neural crest cells

Neuronal cells used in in vitro research are often derived via directed differentiation of human-induced pluripotent stem cells (iPSCs). In order to make sure the iPSCs differentiate into neuronal cells, it is very important to keep a close eye on your cells during this costly 30-day process. CytoSMART’s live-cell imaging microscopes and Anatomic's 7-day neural differentiation method can speed up this process.

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PRESS RELEASES

Announcing the automated organoid counter

CytoSMART Technologies today announced the launch of an automated organoid counter. The software application detects organoids using brightfield image analysis and runs on the Corning® Cell Counter, a CytoSMART product.

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