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Producing organoids in the lab is dependent on the goals of the researcher, and several considerations will be discussed below. How to culture organoids, characterize them and their clinical potential
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CytoSMART devices are connected to the Microsoft Azure cloud environment. In this article, we discuss cloud safety, privacy, and international compliance.
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Although colony formation assays are more widely known as a cancer biology assay, they do have applications in the field of stem cell biology where the self renewal potential of stem cells and their progenitors is assessed. Colony forming assays that are used in this context are not end-point based and rely on live cell imaging. The assay enables selection of desired colonies for further culturing and experimentation. Assessing colony formation is most commonly used for hematopoietic stem cells (HSCs), in the form of a colony formation unit (CFU) assay.
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Working in the lab after COVID-19: remote monitoring of cell cultures as a helpful alternative to optimize your time
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Spheroids have become an exciting cell culture model, as they better mimic in vivo tissue properties and exhibit a superior capacity for differentiation compared to 2D culture systems. These properties make them well suited for use in drug development and tissue engineering studies, as well as for the study of fundamental biological principles.
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In this article we will go into how the proliferative rate and confluency of cells (1) affect baseline gene expression levels, and consequently, the biological interpretation of those data, and (2) influence the efficiency of gene reprogramming and the quality of the resultant products.
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CPE is a very basic approach to understand how a virus infects a cell, but that does not mean it is just used in basic scientific research. Measuring CPEs can also be a very useful readout for pharmaceutical companies and diagnostic laboratories.
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Establishment of protocols for the extraction or differentiation of neural stem cells (NSCs) and their expansion in vitro was a great advancement in neurobiology. NSCs grown in culture provide a powerful tool and an accessible model to investigate human neurodevelopment and cell biology. This article discusses neural stem cell sources, isolation, culture methods and differentiation potential.
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A look into their definition, culturing methods, and therapeutic potential
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In this article we will discuss the clinical use, isolation and culturing specifically for hUC-MSCs. It will become clear that maintain a strict culturing protocol, sticking to specific seeding densities, media and observing confluence is essential to maintain stemness of the MSCs.
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