Resources
CYTOSMART ACADEMY
In this presentation we will explain what the different biosafety levels (BSL) are in a lab. We will explain in depth what the different safety measures are in these biosafety levels.
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Cell migration is an essential process involved in important physiological processes such as embryogenesis, angiogenesis, wound healing and the immune response. Cells migrate in different modes depending on the type and function of the cells. Leukocytes, for example, migrate the majority of their life span as single cells within virtually any tissue in the body. Many other cell types only move at specific situations to place, shape or repair tissue. Most of these cells move in groups that are loosely or closely associated. This type of migration is called collective cell migration. In this article, different modes of both single cell migration and collective cell migration are discussed.
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PromoCELL and CytoSMART experiment: Kinetic analysis of in vitro wound closure by keratinocytes application.
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The wound-healing assay is a convenient and economical method to investigate collective cell migration under different experimental conditions. As collective cell migration is linked to many physiological and pathological processes related to embryogenesis, wound repair and cancer metastasis, the wound healing assay is broadly applicable. We discuss how to set up an experiment and several options to analyze imaging data.
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Producing organoids in the lab is dependent on the goals of the researcher, and several considerations will be discussed below. How to culture organoids, characterize them and their clinical potential
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CytoSMART devices are connected to the Microsoft Azure cloud environment. In this article, we discuss cloud safety, privacy, and international compliance.
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Although colony formation assays are more widely known as a cancer biology assay, they do have applications in the field of stem cell biology where the self renewal potential of stem cells and their progenitors is assessed. Colony forming assays that are used in this context are not end-point based and rely on live cell imaging. The assay enables selection of desired colonies for further culturing and experimentation. Assessing colony formation is most commonly used for hematopoietic stem cells (HSCs), in the form of a colony formation unit (CFU) assay.
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Working in the lab after COVID-19: remote monitoring of cell cultures as a helpful alternative to optimize your time
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Spheroids have become an exciting cell culture model, as they better mimic in vivo tissue properties and exhibit a superior capacity for differentiation compared to 2D culture systems. These properties make them well suited for use in drug development and tissue engineering studies, as well as for the study of fundamental biological principles.
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In this article we will go into how the proliferative rate and confluency of cells (1) affect baseline gene expression levels, and consequently, the biological interpretation of those data, and (2) influence the efficiency of gene reprogramming and the quality of the resultant products.
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