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PromoCell and CytoSMART experiment: Wound Closure Assay
PromoCELL and CytoSMART experiment: Kinetic analysis of in vitro wound closure by keratinocytes application.
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Wound healing assay - what, why and how
The wound-healing assay is a convenient and economical method to investigate collective cell migration under different experimental conditions. As collective cell migration is linked to many physiological and pathological processes related to embryogenesis, wound repair and cancer metastasis, the wound healing assay is broadly applicable. We discuss how to set up an experiment and several options to analyze imaging data.
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Organoids: definition, culturing methods and clinical applications
Producing organoids in the lab is dependent on the goals of the researcher, and several considerations will be discussed below. How to culture organoids, characterize them and their clinical potential
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Clonogenic (colony formation) assay for stem cells
Although colony formation assays are more widely known as a cancer biology assay, they do have applications in the field of stem cell biology where the self renewal potential of stem cells and their progenitors is assessed. Colony forming assays that are used in this context are not end-point based and rely on live cell imaging. The assay enables selection of desired colonies for further culturing and experimentation. Assessing colony formation is most commonly used for hematopoietic stem cells (HSCs), in the form of a colony formation unit (CFU) assay.
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Remote working in a biotech lab
Working in the lab after COVID-19: remote monitoring of cell cultures as a helpful alternative to optimize your time
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Spheroids: properties, image analysis and culture methods
Spheroids have become an exciting cell culture model, as they better mimic in vivo tissue properties and exhibit a superior capacity for differentiation compared to 2D culture systems. These properties make them well suited for use in drug development and tissue engineering studies, as well as for the study of fundamental biological principles.
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Gene expression, gene editing and cell confluency: how cell culture parameters can make or break your studies
In this article we will go into how the proliferative rate and confluency of cells (1) affect baseline gene expression levels, and consequently, the biological interpretation of those data, and (2) influence the efficiency of gene reprogramming and the quality of the resultant products.
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Virus-induced cytopathic effect (CPE): examples and readouts
CPE is a very basic approach to understand how a virus infects a cell, but that does not mean it is just used in basic scientific research. Measuring CPEs can also be a very useful readout for pharmaceutical companies and diagnostic laboratories.
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Neural Stem Cells
Establishment of protocols for the extraction or differentiation of neural stem cells (NSCs) and their expansion in vitro was a great advancement in neurobiology. NSCs grown in culture provide a powerful tool and an accessible model to investigate human neurodevelopment and cell biology. This article discusses neural stem cell sources, isolation, culture methods and differentiation potential.
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Human umbilical cord mesenchymal stem cells
In this article we will discuss the clinical use, isolation and culturing specifically for hUC-MSCs. It will become clear that maintain a strict culturing protocol, sticking to specific seeding densities, media and observing confluence is essential to maintain stemness of the MSCs.
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