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Label-free live cell imaging versus fluorescent live cell imaging - CytoSMART Academy

Here we compare label-free live cell imaging versus fluorescent live cell imaging. Although both techniques have become extremely important in the field of cell culture, there are certain threats to consider when using them. While label-free live cell imaging is non-invasive it is harder to visualise specific structures and proteins. Also, the preparation time and skills of the researcher need to be taken into consideration as fluorescent live cell imaging is more time consuming, is high in costs and is not easy to set-up. In addition, because of it’s non-invasiveness, label-free live cell imaging can be used with a higher temporal resolution without changing the culture conditions.

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