Cell migration is an essential aspect of a variety of physiological processes, such as embryogenesis, angiogenesis, wound healing, and the immune response. Unfortunately, cell migration is also involved in many pathologies, including cancer metastasis and inflammation. In each of these physiological and pathological processes, the basic process of cell migration, i.e. translocation along or through a tissue substrate, is the same. However, cells migrate in different modes depending on the type and function of the cells. Leukocytes, for example, migrate the majority of their life span as single cells within virtually any tissue in the body. Many other cell types only move in specific situations to place, shape, or repair tissue. Most of these cells move in groups that are loosely or closely associated. This type of migration is called collective cell migration.
In this e-book, we aim to provide tools to help you find the most suitable experimental setup and corresponding analysis method to investigate collective cell migration.
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