When a stem cell culture gets too confluent, there is a risk that stem cells will start differentiating into other cell lineages or will stop proliferating at all and go quiescent. This video describes how the Lux2 (CytoSMART Technologies) can help and improve your stem cell culture. The Lux2 is a live-cell imaging device that fits in any cell culture incubator and creates time-lapse videos in the CytoSMART Cloud. Confluence percentage is no longer estimated but calculated, resulting in an objective and accurate confluence output. Because all videos and images are stored in the Cloud, researchers can remotely monitor their cells (live!) anywhere and at any given time. This reduces the handling steps in the cell culture monitoring process (e.g. taking the cells out of the incubator, checking under a regular microscope, etc), which decreases the chances of stem cell culture contamination. Lastly, confluence percentage notifications can be set in the Cloud, providing the researcher with extra data that helps planning and diminishing the need for daily lab visits.
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