Live-cell imaging allows researchers to determine not only whether, but also how and when certain biological events occur in cell culture. In order to achieve the high image quality standards required for data analysis and publications, live-cell imaging is performed predominantly using a microscope with a stage top incubation box. However, the culture conditions in the incubation box are more prone to fluctuations, as compared to a dedicated cell culture incubator. Using the CytoSMART® Lux3 BR, it is no longer necessary to choose between optimal cell culture conditions and image quality.