CytoSMART Omni

Live-cell imaging
for high-throughput
experiments

  • CytoSMART Omni

CytoSMART Omni

Being able to monitor cell cultures over time provides a great insight into their physiology and function. Live-cell imaging microscopes open up novel and exciting avenues to study cellular health, viability, colony formation, migration, and cellular responses to external stimuli.

To help life science researchers improve their understanding of cellular processes, CytoSMART Technologies has developed an automated bright-field microscope that can visualize an entire surface of a cell culture vessel and operate from inside a standard CO2-incubator, biological safety cabinet or on a benchtop. Not limited to a specific type or quantity of culture vessels, the CytoSMART Omni captures cellular behavior by creating high-quality time-lapse videos for days or even weeks at a time.

Top-end technology that keeps on improving.

Manuel Reitberger German Cancer Research Center (DKFZ)

"Constant development of analysis software in cloud is excellent. Hardware improvements are immediately implemented into the device. Great support by the company."

4.3
Application Area: 癌症研究

Amazing for long-term imaging of whole wells and plates!

Neshika Wijewardhane King's College London

"Very easy product to use. Does require a bit of setup, but the company sends out a representative to help with this. Also, they ask what I want to use the device for and really tailor the experience. Love that it can be used inside an incubator as I had trouble with long-term imaging with on stage incubators for big microscopes. Also, don't need to worry about fluctuations in temperature and oxygen percentages and deal with oxygen tanks. The confluency data is very useful and produces graphs ready for you to use. Maybe have more options for well/plates to be able to scan."

4.3
Application Area: 长期即时成像

Very good and recommended.

Ebrahim Hamza Freie Universität Berlin

"The device was very easy to set up, run experiments in and the data it produces is reliable."

5.0
Application Area: 监测细胞培养物的增殖

Useful tool that allows for fast progression.

Johannes Vandaele KU Leuven

"Compact and nicely-designed piece of equipment. Very straightforward to use in terms of hardware and software."

4.0
Application Area: 监测细胞生长

Continuous imaging provides real-time insight into cellular processes

Perform kinetic assays over the course of days or even weeks

Time-lapse imaging allows you to pinpoint important events in the progression of the cell culture from experiment to experiment. Uncover attachment and detachment rates, evaluate events like cell death and compare growth rates. Data collection using video monitoring allows users to capture changes within samples and compare the rate of change between samples. With the integrated Continue Experiment feature, it is now possible to perform multiple transient screens in parallel to an ongoing long-term kinetic assay by simply pausing the running experiment.

Complete overview of sample confluency

Achieved by automated full plate scanning and image stitching

Manual handling and cell seeding can cause a variable density distribution within culture vessels. Randomly selecting several areas of interest or tile scanning is common practice to overcome that issue, however this is either time-consuming to set-up or to post-process. The CytoSMART Omni automatically scans complete well surface areas and instantly stitches these images to give users a complete overview of cell coverage.

Compatibility with any transparent culture vessel

Imaging of full plates, flasks, dishes and microfluidic devices

Cell based assays and subculturing all benefit from standardization. Choosing the right vessel and going through the process of understanding how cells behave in said vessel is vital for reproducibility. The CytoSMART Omni is highly flexible and is compatible to any transparent vessel that is lower than 55 mm. All vessels with a surface area smaller than 99*131 mm can be imaged in full (6 – 384-well plates, T-25 – T225, Petri dishes, triple flasks, and HYPERFlasks).

Efficient screening of culture multiwell plates

The CytoSMART Omni can scan up to 6 vessels per hour

High-throughput experiments are not limited to a single culture vessel. The CytoSMART Omni can process multiple plates in quick succession, automatically stitching acquired images and safely storing data for each individual plate.

The CytoSMART Omni is ideal to perform kinetic assays to map cell proliferation, which we use as a tool to understand drug resistance in pancreatic cancer cell lines.

Dr. Martin Sprick | Senior Group Leader

Heidelberg Institute for Stem Cell Technology and Experimental Medicine

The CytoSMART  Omni is ideal to perform kinetic assays to map cell proliferation, which we use as a tool to understand drug resistance in pancreatic cancer cell lines.
  • Incubator-friendly
  • Label-free cellular assays
  • Time-lapse videos
  • Image stitching
  • Cloud-based image analysis algorithms

Would you like to test if the CytoSMART Omni is beneficial for your laboratory?

Kinetic assays: cell proliferation, migration,
colony formation & more

Image analysis can be used to extract valuable information from time-lapse videos. Use our cell monitoring software to investigate and compare parameters like cell confluence, area infiltration (wound healing assays) and growth rate.

Perform analysis at desired culturing environment

The CytoSMART Omni is designed for your incubator

Live-cell imaging systems require environmental control during the experiment. To ensure the proper environmental conditions, users can place the compact CytoSMART Omni inside any standard cell culture incubator. The smooth curvatures of the CytoSMART Omni platform ensures minimal airflow disturbance. This ensures that your samples stay in their desired, homogeneous environment throughout the entire experiment. What's more, the versatile nature of the CytoSMART Omni allows it to be used directly at a bench or biological safety cabinet, ensuring the most optimal setting for conducting an imaging experiment.

Ensure sample stability while imaging

Produce bright-field images without moving your samples

Acquiring images can be highly disturbing to living cells. Environmental shock, like sudden changes in the atmosphere and mechanical disturbance can negatively influence cell viability. The CytoSMART Omni rapidly captures bright field images at cell-level detail without disturbing the cells. Users can set the desired imaging parameters using their own computer and the CytoSMART Omni will follow that scanning regime. There is no need to move a culture vessel on the sample stage, since the camera moves along the platform to capture the entire area of the vessel. Automated time-lapse imaging eliminates the need for any manual interference. Imaging using such a stable system prevents unwanted disturbances and reduces imaging artifacts.

Technical specifications

Unit dimensions 396 x 345 x 171 mm (L x W x H)
Weight 9 kg
Optics Bright-field with digital phase contrast
Magnification 10x fixed objective
Light source LED
Camera 5 MP CMOS
Scan area 99 * 131 mm
Exported formats JPG, XLSX & MP4
Well plate types 6 - 96 well plates
Culture flask types petri dishes, T25 - T225, triple flasks and HYPERFlasks
Other labware Anything transparent and lower than 55 mm
Operating environment 5-40 °C, 20-95% humidity
Support Via email and live chat
Research use only. Not intended for diagnostic purposes

Videos

Appnotes

Cell Viability Analysis

Comparison Between Metabolic Activity Assay and Confluence Analysis
Download Appnote

Cell Cytoxicity Assay to Analyze Drug Response

Evaluating Drug Toxicity Using Label-Free Microscopy
Download Appnote

Cell Migration

Live-Cell Imaging and Quantitative Analysis of Wound Healing Assays
Download Appnote

Frequently Asked Questions

Inverted bright field microscopy is used and images are enhanced using digital phase contrast. The camera moves below the sample stage. Samples are illuminated using LED lighting and a scanning motion, which facilitates sequential image generation. One complete scan takes 6 minutes in which 6500 images are generated. These are captured and stitched to form an image of a 99*131 mm surface area. The images are uploaded to the CytoSMART™ Cloud, there they can be analyzed using our image analysis algorithms.

You can specify the interval rate between 1 - 24 h or choose to perform a single scan.

The magnification is equivalent to 10X objective of a traditional bright field microscope

Confluence, scratch analysis and colony detection are currently part of the image analysis software package. Users always have the option to download the raw image data and perform their own analysis.

Yes, the CytoSMART Omni is designed to be used inside a cell culture incubator. Its hardware and electronics can operate at 5 - 40 °C and between 20 - 95% humidity.

No, our image analysis algorithms are optimized to be used in label-free assays, so you don’t have to add (toxic) dyes to your cells, providing a non-invasive analysis of your cells.

Yes, the device can only be used with a Windows-based computer with a USB 3.0 port (which can also be purchased at CytoSMART™). A WiFi or wired ethernet connection is necessary to be able to connect to the CytoSMART™ Cloud for data storage and analysis.

Any culture vessel that is lower than 55 mm (height of the light arc) can be scanned. However, the size of the scan is limited to 99*131 mm, which fits a complete T175 flask.


Some examples include 6-96 well plates, petri dishes, T25 - T225, triple flasks and HYPERFlasks.

Yes, after sterilizing with ethanol (70%) or IPA, the device can be used in a cleanroom. Do not use Acetone to clean the device, also the device cannot be autoclaved.

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