CytoSMART Omni

Capture cellular behavior
using live-cell imaging

  • CytoSMART Omni

CytoSMART Omni

Being able to monitor cells over time, provides great insight in their dynamics and function. Video monitoring opens up novel and exciting avenues, to study cell health and viability, colony formation, migration and cellular responses to external factors.

To help researchers in life sciences to continue improving their understanding of cellular processes, CytoSMART has developed an automated bright-field microscope that visualizes complete culture vessels and operates from inside standard CO2-incubators. Perform kinetic assays that capture cellular behavior for days or even weeks at a time.

Continuous imaging provides real-time insight into cellular processes

Perform kinetic assays over the course of days or even weeks.

Pinpoint important events in the progression of the cell culture from experiment to experiment. Uncover attachment and detachment rates, elvaluate events like cell death and compare growth rates. Data collection using video monitoring allows users to capture changes within samples and compare the rate of change between samples.

Complete overview of sample heterogeneity

Achieved by automated full plate scanning and image stitching

Manual handling and cell seeding can cause a variable density distribution within culture vessels. Randomly selecting several areas of interest or tile scanning is common practice to overcome that issue, however this is either time-consuming to set-up or to post-process. The CytoSMART Omni automatically scans complete well surface areas and instantly stitches these images to give users a complete overview of cell coverage.

High throughput assays or cell line production

Imaging of full plates, flasks, dishes and microfluidic devices

Cell assays and subculturing all benefit from standardization. Choosing the right vessel and going through the process of understanding how cells behave in said vessel is vital for reproducibility. The CytoSMART Omni is highly flexible and is compatible to any transparent vessel that is lower than 55 mm. All vessels with a surface area smaller than 99*131 mm can be imaged in full.

Visualizations from overview to cell level detail.

The CytoSMART Omni generates 6500 images at cell level detail; 10X magnification, 6 minutes for a full plate. These images are automatically stitched and provide a complete overview of the entire vessel that is used during assays and culturing.

  • Label-free cellular assays
  • Time-lapse videos
  • Whole well surfaces

Would you like to test if the CytoSMART Omni is beneficial for your laboratory?

The CytoSMART Omni is ideal to perform kinetic assays to map cell proliferation, which we use as a tool to understand drug resistance in pancreatic cancer cell lines.

Dr. Martin Sprick | Senior Group Leader

Heidelberg Institute for Stem Cell Technology and Experimental Medicine

The CytoSMART  Omni is ideal to perform kinetic assays to map cell proliferation, which we use as a tool to understand drug resistance in pancreatic cancer cell lines.
Understand Proliferation, Migration,
Colony counting & more.

Image analysis can be used to extract valuable information from time-lapse video's. Investigate and compare parameters like confluence, area infiltration and growth rate.

Perform analysis at desired culturing environment

The CytoSMART Omni is designed for your incubator

Live-cell imaging requires environmental control during the experiment. To ensure the proper environmental conditions, users can place the compact CytoSMART Omni inside any standard cell culture incubator. The smooth curvatures of the CytoSMART Omni platform ensures minimal airflow disturbance. This ensures that your samples stay in their desired, homogeneous environment throughout the entire experiment.

Ensure sample stability while imaging

Produce bright-field images without moving your samples

Acquiring images can by highly disturbing to living cells. Environmental shock, like sudden changes in the atmosphere and mechanical disturbance can negatively influence cell viability. The CytoSMART Omni rapidly captures bright field images at cell-level detail without disturbing the cells. Users can set the desired imaging parameters using their own computer and the CytoSMART Omni will follow that scanning regime. At no point after placing the cells on the sample stage does the culture need to be moved. Automated time-lapse imaging eliminates the need for any manual interference. Imaging using such a stable system prevents unwanted disturbances and reduces imaging artifacts

Technical specifications

Unit dimensions 396 x 345 x 171 mm (L x W x H)
Weight 9 kg
Optics Bright-field with digital phase contrast
Magnification 10x fixed objective
Light source LED
Camera 5 MP CMOS
Scan area 99 * 131 mm
Exported formats JPG, XLSX & MP4
Well plate types 6 - 96 well plates
Culture flask types petri dishes, T25 - T225, triple flasks and HYPERFlasks
Other labware Anything transparent and lower than 55 mm
Operating environment 5-40 °C, 20-95% humidity
Support Via email and live chat
Research use only. Not intended for diagnostic purposes

Videos

Appnotes

Cell Viability Analysis

Comparison Between Metabolic Activity Assay and Confluence Analysis
Download Appnote

Cell Cytoxicity Assay to Analyze Drug Response

Evaluating Drug Toxicity Using Label-Free Microscopy
Download Appnote

Cell Migration

Live-Cell Imaging and Quantitative Analysis of Wound Healing Assays
Download Appnote

Frequently Asked Questions

Inverted bright field microscopy is used and images are enhanced using digital phase contrast. The camera moves below the sample stage. Samples are illuminated using LED lighting and a scanning motion, which facilitates sequential image generation. One complete scan takes 6 minutes in which 6500 images are generated. These are captured and stitched to form an image of a 99*131 mm surface area. The images are uploaded to the CytoSMART™ Cloud, there they can be analyzed using our image analysis algorithms.

You can specify the interval rate between 1 - 24 h or choose to perform a single scan.

The magnification is equivalent to 10X objective of a traditional bright field microscope

Confluence, scratch analysis and colony detection are currently part of the image analysis software package. Users always have the option to download the raw image data and perform their own analysis.

Yes, the CytoSMART Omni is designed to be used inside a cell culture incubator. Its hardware and electronics can operate at 5 - 40 °C and between 20 - 95% humidity.

No, our image analysis algorithms are optimized to be used in label-free assays, so you don’t have to add (toxic) dyes to your cells, providing a non-invasive analysis of your cells.

Yes, the device can only be used with a Windows-based computer with a USB 3.0 port (which can also be purchased at CytoSMART™). A WiFi or wired ethernet connection is necessary to be able to connect to the CytoSMART™ Cloud for data storage and analysis.

Any culture vessel that is lower than 55 mm (height of the light arc) can be scanned. However, the size of the scan is limited to 99*131 mm, which fits a complete T175 flask.


Some examples include 6-96 well plates, petri dishes, T25 - T225, triple flasks and HYPERFlasks.

Yes, after sterilizing with ethanol (70%) or IPA, the device can be used in a cleanroom. Do not use Acetone to clean the device, also the device cannot be autoclaved.