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CytoSMART Lux3 FL Duo Kit

Fluorescence live-cell imaging
for side-by-side
comparative studies

  • CytoSMART Lux3 FL Duo Kit

Fluorescence live-cell imaging allows life scientists not only to monitor the quality of cell cultures, but also to track complex biological processes with a higher degree of spatial and temporal resolution.

The CytoSMART Lux3 FL Duo Kit is a compact and cost-effective imaging system consisting of two dual-fluorescence microscopes that is designed for systematic comparison of cell cultures. The system can operate from within a standard CO2-incubator or a hypoxia chamber, allowing to analyze cells in their desired culture environment. Two devices operate via a single laptop, maximizing laboratory space. The imaging of samples under identical cell culture environment provides a robust platform for unambiguous comparison between different treatments and cell culture conditions. The images and time-lapse movies can be accessed and analyzed remotely via the CytoSMART Cloud, providing real-time updates on the status of running experiments.

  • Versatile – expand the number of variables you can examine using fluorescent labeling
  • Incubator-friendly – study cells in their desired culture environment
  • Ideal for comparative studies – directly compare fluorescently-labeled cell cultures
  • Cost-effective and efficient solution – save money and time performing cell culture experiments

Dual-fluorescence and brightfield imaging of dynamic cellular processes

Analyze multiple cell features using brightfield and fluorescence microscopy

Label-free (brightfield) and labeled (fluorescence) imaging of live cells can provide a wealth of valuable biological information on cellular dynamics, morphology, and structure. The CytoSMART Lux3 FL Duo Kit combines brightfield imaging with green and red fluorescence, and makes it possible to study multiple cell features or cell types, among many other things. The integrated image analysis software provides immediate insight into the confluence levels of fluorescently-tagged cells, as well as the exact number of fluorescent objects in the image. The Lux3 FL Duo Kit can be used for a variety of applications, including analysis of cell viability and cellular processes, single-cell migration, and co-culture models.

Side-by-side comparison of cell cultures

Compare different cell culture conditions, controls, and treatments

Duo flux 3

The CytoSMART Lux3 FL Duo Kit is an ideal tool for systematic in vitro comparative studies of cell cultures. The Duo Kit fits comfortably inside a standard cell culture incubator or a hypoxia chamber, which allows to maintain cell cultures in an identical environment. As a result, the growth and viability of cells subjected to different culture conditions (e.g. growth medium, pH) or treatments, can be compared against each other and positive and negative controls in a direct and unbiased manner. This comparison can also be measured quantitatively via the Object Count algorithm that can automatically calculate the number of fluorescent objects in a tested culture vessel and track how this number changes over time.

Technical specifications

Number of Lux3 FL units 2
Channels Brightfield, green, and red fluorescent channels
Peak wavelength / bandwidth Green – excitation: 452/45 nm; emission: 512/23 nm
Red – excitation: 561/14 nm; emission: 630/90 nm
Magnification 10× (digital zoom 20×)
Image resolution 2072 × 2072 pixels
Field of view (per device) 1.45 mm × 1.45 mm
Camera 6.4 MP CMOS
Light source LED
Data formats JPG, TIFF, MP4, XLSX
Data storage Unlimited cloud storage & unlimited number of user accounts
Computer requirements Windows 10, two USB3 ports 2.4 GHz i5 processor, 4 GB RAM memory, internet
Culture vessels Any transparent culture vessel: well-plates, petri dishes, flasks, microfluidic chips, and custom culture vessels
Dimensions (L × W × H; per device) 16.6 cm × 14 cm × 13.5 cm
Weight (per device) 1.3 kg
Operating conditions 5 – 40 °C, 20 – 95% humidity
Warranty 1-year parts and labor
Extra features Template functionality
Object count
Research use only. Not intended for diagnostic purposes

Frequently Asked Questions

The CytoSMART Lux3 FL Duo Kit is a live-cell imaging system that employs two compact fluorescence microscopes, designed to be placed inside an incubator to monitor cell cultures. The CytoSMART Cloud environment provides real-time updates on the status of running experiments and allows to access images and time-lapse videos remotely.

If you already have a single CytoSMART Lux3 FL device in your lab, upgrading to the Duo Kit can be done by simply ordering an extra CytoSMART Lux3 FL. Both devices can operate via a single laptop (or desktop), as long as the laptop has two available USB 3.0 ports.

The CytoSMART Lux3 FL Duo Kit is compatible with a wide range of different culture vessels, including T-flasks (T-25 to T-250), multi-well plates (6 – 384-well plates), microfluidic chips, flat tubes, petri dishes, and slides.

The remote functionality of the CytoSMART Lux3 FL Duo Kit runs on cloud-based computing. In this cloud environment, the images and videos are stored and can be accessed using user-specific login details. Next to unlimited data storage and unlimited number of user accounts, automated image analysis can be performed in the CytoSMART Cloud portal.

The devices are easy to clean using lint-free wipes and ethanol (70%) or isopropyl alcohol (IPA). Do not use acetone to clean the devices. Please be aware that the devices cannot be autoclaved.

After sterilizing with ethanol or IPA, the devices can be used in a clean room.

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