Cyto SMART Lux2 - Duo Kit - hero

of cell cultures

CytoSMART Lux2 Duo kit

Live-cell imaging is a useful tool for monitoring the quality of cell cultures. A simple but effective set-up utilizing a high quality camera allows researchers to evaluate cellular health at regular points in time. The CytoSMART Lux2 duo-kit is a compact and cost-effective method for carrying out this type of analysis, following the complete growth progression of mammalian cells.

The CytoSMART Lux2 Duo Kit is an compact automated system, specifically designed to operate from within CO2- incubators and hypoxia chambers. Two devices operate via a single laptop, saving precious lab space. Gathering real-time insight into the progression of cell growth is now completely non-invasive. Bright-field imaging is deployed to create real time time-lapse videos, accessible remotely. Samples are also imaged under identical conditions, providing a robust platform for unambiguous comparison between cell culture variables, retaining data integrity.

Deploying this dual camera mini live cell imaging system is particularly useful in instances of stem cell research. The remote functionality of the CytoSMART cloud allows researchers to gain insight into the current confluence levels on their phone. This type of microscopy avoids over-growing of cultures or the loss of stemness in the cell-line, ensuring optimal yield. Your team can inspect the colonies that are formed by iPSCs and the morphology of MSCs without setting foot in the lab. Reducing the time spent in the lab will be crucial for many groups that try to continue research while complying to the social distancing rules

The CytoSMART Lux2 duo kit enables us to monitor the cells in our microfluidic chip real-time and from home, which gives us valuable information about our chip functionality, even when we are not physically present in the laboratory

Dr. Sandra Hofmann | Associate Professor in Bioengineering Bone

Eindhoven University of Technology

The CytoSMART Lux2 duo kit enables us to monitor the cells in our microfluidic chip real-time and from home, which gives us valuable information about our chip functionality, even when we are not physically present in the laboratory

Inspect morphology and analyze cell confluency

Compare cell cultures, supported by robust image analysis software

Imaging at regular time-intervals allows researchers to make quick decisions around cell quality, as any negative indicators such as blebbing, cell rounding, multi-nucleation and vacuole formation can be easily recognized. Variability in growth speed is easily recognized using the CytoSMART cell monitoring software, while image analysis provides an unambiguous read out for cell confluency meaning you can be confident in your data’s integrity. As such, the system is the ideal approach for the systematic comparison of cultures, in order to select the ideal culturing conditions.

Keep cells in a controlled environment

Complete cell culture incubator compatibility

Cyto SMART Lux2 - Duo Kit

During the observation of cell cultures, the right environmental conditions are vital in order to maintain cell health and keep cells active. Using a remote system avoids exposing the cells to potential mechanical and environmental shock from removing them from the incubator. Once the samples are placed inside the incubator, they no longer need to be handled. The researcher can check videos of the cell cultures in real-time, providing immediate insight into the culture quality. No need to step into the lab for routine inspection rounds, making the process efficient, cost effective and safe.

Technical specifications

OpticsBrightfield with digital phase contrast
Magnification10× fixed objective – 20× digital zoom
Fluorescence filtersN/A
Camera5.0 MP CMOS
Data formatsJPG, XLSX, MP4
Image size960 × 960 pixels
Field of view1.84 mm × 1.84 mm
Culture vesselsWell-plates, petri dishes, flasks, microfluidic chips, and custom culture vessels
Computer requirementsWindows 10, two USB3 ports 2.4 GHz i5 processor, 4 GB RAM memory, internet
Power supplyAC 100-240V, 2A, 10W, 50/60HZ
Dimensions (L × W × H)133 mm × 90 mm × 100 mm
5.2 in × 3.5 in × 3.9 in
Weight0.5 kg (1.1 lb)
Operating conditions0 – 42 °C, 5 – 95% humidity
Warranty1-year parts and labor
Data storageUnlimited cloud storage
Research use only. Not intended for diagnostic purposes

Frequently Asked Questions

If you already have a single CytoSMART Lux2 in your lab, upgrading to the Duo-Kit can be done by simply ordering an extra CytoSMART Lux2. Any software updates can be performed remotely. No extra laptop is needed. An additional USB3 port should be available.

The Duo kit is a device, which employs two compact inverted microscopes that are designed to be placed inside an incubator, to monitor cell cultures. The system can be used as a notification system to alert users when a cell culture confluence target is reached.

No calibration by the user is needed for the devices to operate.

The system requires a dedicated desktop or a laptop running on Windows 10 or above, with two USB3 ports and an active internet connection. If your lab doesn’t have the required hardware, we can supply these for a fee as part of your order.

The CytoSMART Lux2 remote functionality runs on cloud-based software. In this cloud-environment the images are stored and can be accessed using user-specific login details. Next to unlimited data storage, image analysis algorithms can be run to quantify confluence and cell migration.

The CytoSMART Lux2 Duo Kit allows monitoring of a wide range of different culture dishes and flasks, such as:

  • ​T-flasks: T-25 up to T-250

  • Single well, multi-well plates (6 - 384-well plates)

  • Microfluidic chips

  • Flat tubes

  • Petri dishes

  • Slides

This is not an exhaustive list, so if your preferred equipment isn’t on this list, please get in touch with us. For larger flasks an adapter plate may be required. This plate can be purchased at CytoSMART.

Magnification is equal to a standard microscope with a 10x fixed objective and 20x digital zoom.

Images can be recorded at pre-defined intervals. At the start of a new project, you can specify the interval rate anywhere between 5 min - 12 hours.

The devices are easy to clean using lint-free wipes and ethanol (70%) or isopropyl alcohol (IPA). Do not use acetone to clean the device. Please be aware that the device cannot be autoclaved.

After sterilizing with ethanol or IPA, the device can be used in a clean room.

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